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"Plant Tissue
Culture Propagation" CD

1993 edition with 2007 update

 

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Publication: Table of Contents

 

Plant Tissue Culture Propagation (1993)

xvi + 185 + 872 colour photographs

 

Preface

Introduction

Preparation Area

  • Preparation of Concentrated Chemical Stock Solutions
  • Preparation of Culture Media
  • Alternative Methods of Sterilizing Culture Media
    • Membrane Sterilization
    • Sterilization with Organic Solvents
  • Some Other Substances Added to Culture Media
  • Powdered Culture Media

Inoculation Area

  • Inoculation Technique: Aseptic Procedures
  • Warning: Fire Hazard

Incubation Area

Disinfestation

  • Physical Methods
  • Chemical Methods
  • Summary of Standard Disinfestation Treatment
  • Recovering Cultures Contaminated with Microbes

Mini Broad Spectrum Experiment-I

Mini Broad Spectrum Experiment-II

Step by Step Procedures to Achieve Clonal

Propagation of a Cultivar

  • Pathway-I: Experimentation with Aseptic Seedlings
  • Pathway-II: Initial Culture and Growth of Selected Genotype

Clonal Propagation of Strawberry

Eucalyptus Research

  • Eucalyptus Callus
  • Pathway-I:- Organ Culture with Eucalyptus Seedling Material
  • Pathway-II: - Organ Culture with Adult Eucalyptus Trees
  • Present Direction of Eucalyptus Research

Eriostemon Research

Initiation of Tissue Cultures of a Range of Plant Types

  • Dioscorea Discolor (Dioscoreaceae)
  • Jewel Orchid (Haemaria Discolor X "Dawsoniana") (Orchidaceae)
  • Saxifraga Sarmentosa (Saxifragaceae)
  • Syngonium Podophyllum (Araceae)
  • Maranta Leuconeura Erythrophylla (Marantaceae)
  • Jojoba (Simmondsia Chinensis) (Simmondsiaceae)
  • Hyacinthus Orientalis (Liliaceae)
  • African Violet (Saintpaulia Ionantha) (Gesneriaceae)
  • Begonia Rex-Culturum (Begoniaceae)

Tissue Culture Propagation of Grevillea

Tissue Cultures of a Number of Species

  • Cauliflower (Brassica oleracea var. botrytis) (Cruciferae)
  • Hoop Pine (Araucaria cunninghamii) (Araucariaceae)
  • Miscellaneous Cultures

Orchid Cultures

  • Immature Seed Culture of a Sympodial Orchid
  • Initiation of Clonal Propagation of a Cymbidium
  • Initiation of Clonal Propagation of a Lealiocattleya
  • Broad Spectrum Experimentation with Cymbidium Cultivars
  • Induction of Polyploidy with Colchicine
  • Protocol for Chromosome Counting
  • Measuring Guard Cells to Detect Polyploidy
  • Tissue Cultured Orchid Plants Established in Potting Mix

Ferns

  • Aseptic Culture of Fern Spores
  • Clonal Propagation of Nepholepis Ferns

Planting out of Tissue Cultured Plants

  • Defining the Problems Associated with Planting Out
    • Desiccation
    • Microbial Contamination
    • Photosynthesis
    • Rooting

Production Costs of Tissue Cultured Plants

Virus Eradication

  • Strategy to Eradicate Viruses by a Combination of Heat Treatment and Meristem Culture

Tobacco Callus Research

Anther Culture and Haploid Sporophytes

  • Tobacco Anther Culture
  • Rice Anther Culture
  • Sunflower Anther Culture

Bibliography

Index

About the Author

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2007 addition - Table of Contents

 

Plant Tissue Culture Propagation 2007

iii + 95 + 2 plates + 35 colour photographs

 

Introduction

  • Layout of Laboratory
  • Entrace and Hallway
  • Office/Study
  • Preparation Area
  • Preparation of Concentrated Chemical Stock Solutions
  • Cold Dispensing of Culture Medium
  • Inoculation Room and Laminar Flow Layout
  • Incubation Rooms
  • Lights and Shelving for Cultures
  • Which Species are incubated in Each Room?
  • Now for a Really Large Scale Micropropagation Business

Finding Suitable Culture Media

  • Broad Spectrum Experimentation
  • Exploratory Experiment-I
    • Objective:
    • To establish the most promising concentrations of minerals and sucrose for a species so as to have a good Basal medium for later experimentation.
  • Exploratory Experiment-II
    • Objective:
    • To establish the most promising concentrations of auxins and cytokinins for a species using the new selected Basal Medium from EXPLORATORY EXPERIMENT-I

MICROPROPAGATION AND CONTAMINATION

  • Working Hypothesis No.1
    • Contamination is caused by poor technique, by poor hygiene in the laboratory or by faulty autoclaving.
  • Working Hypothesis No.2
    • Population of microbes is too large to be controlled by prophylactic control methods
    • Development of Disinfection Protocols
    • Devleopment of Disinfection Protocols for Late Contamination
    • Conclusion
  • Working Hypothesis No.3
    • Chlorine treatments are capable of killing all microbes except under certain conditions. If, after chlorine treatment, contamination occurs, it is because the chlorine has failed to make contact with the microbes for long enough. Or contamination may occur if the chlorine has been denatured in some way (such as by contact with organic matter).
  • Disinfestation with Pool Chlorine
  • An Iodometric Method for Measuring Available Chlorine Content of Hypochlorite Solutions
    • Reagents
    • Procedure to Estimate Available Chlorine Content of a Solution
    • Calculation of Available chlorine Concentration in Test Solution
    • Note from PLANT-TC LIST (17 February 1999)
  • Working Hypothesis No.4
    • Contamination of cultures, after numerous passages, is either due to long hidden microbes (low initial population and/or unsuitability of culture medium) or to the isolation and nurturing of chlorine-resistant strains of microbes.
    • Summary of Possible Mechanisms of Drug-Resistance
    • Resistance to Penicillin
  • Working Hypothesis No.5
    • Plants in culture produce antibiotics capable of inhibiting the growth of microbes. This postulated antibiotic production is maximal with vigorously growing cultures but subculturing reduces the volume of plant material (and, hence, antibiotic) leading to contamination in apparently aseptic donor cultures. Any factor limiting/reducing the growth of cultures leads to a concomitant reduction in antibiotic production releasing inhibition exerted on any microbes present.
  • Working Hypothesis No.6
    • The role of ingredients in culture media relative to microbial contamination
    • Sucrose
    • Salt
    • Ingredients in media used for micropropagation
  • Working Hypothesis No.7
    • There is an inbuilt destiny to cultures that determines their longevity. After a predetermined number of passages their growth declines, they age and in consequence, are at the mercy of many types of hazards.
  • Concluding Working Hypothesis
    • Currently, we have a four-pronged strategy
  • A review of Some Pertinent Literature

Mites

  • What are mites?
  • The Pure Science Approach to Problems with Pests
  • Prophylactic suggestion from the Factory (Laboratory) floor
  • What do we do with Mites in our Laboratory?
  • Final Words

Major Research Work

  • THE UNIVERSITY of EDINBURGH
    • What Happened after Graduation at Edinburgh?
  • THE MINISTRY of AGRICULTURE and LANDS, JAMAICA
    • Post-Harvest Physiological Disorder of Oranges
  • SOUTH AFRICA
    • The Citrus & Subtropical Fruit Research Station , Nelspruit, Transvaal
    • Department of Botany, University of the Witwatersrand, Johannesburg
    • Low Volume Spraying
    • Citrus Viruses
  • NEW SOUTH WALES, AUSTRALIA
  • 1961-1964: Divsion of Food Preservation, Commonwealth Scientific & Industrial Research Organisation (CSIRO Food Preservation), NORTH RYDE;
  • 1964-1979: Department of Botany, The University of New England, ARMIDALE
    • FLOWERING in vitro and a POTENTIAL BIOASSAY for FLORIGEN

The Development of New Techniques and Methods

  • Glass and Plastic Culture Vessels
  • The Addition of Sterilized Chemicals and Plant Extracts to Autoclaved Culture Media
  • Home-made Tungsten Wire Needles for Fine Dissections such as Apical Meristems
  • "Sausages" for the Incubation of Cultures
  • Analysis of Culture Media and Use of Mole Units
  • The Broad Spectrum Tissue Culture Experiment
  • Cloning of Eucalyptus Using Tissue Culture
  • Haploid Plants
  • Histochemistry
  • Blue-Green Algae
  • Non-Botanical Activities

Text Books on Clonal Micropropagation

Sundry Publications

Bibliography